目的 建立银杏叶药材中白果新酸(C13∶0)、白果酸(C15∶1)、十七烷二烯银杏酸(C17∶2)、氢化白果酸(C15∶0)和十七烷一烯银杏酸(C17∶1)等5种银杏酚酸类成分的含量测定方法。方法 采用HPLC法。色谱柱为Phenomenex Luna C18(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-0.1%磷酸(90∶10)为流动相,流速为1.0 mL·min-1,检测波长为310 nm,柱温为30 ℃。结果 白果新酸(C13∶0)、白果酸(C15∶1)和十七烷一烯银杏酸(C17∶1)质量浓度分别在1.47~29.40、6.05~121.00、8.00~160.00 μg·mL-1内呈良好线性关系,相关系数分别为0.999 9、0.999 9和0.999 9,平均回收率分别为98.6%(RSD=2.57%,n=9)、100.1%(RSD=2.36%,n=9)、97.4%(RSD=2.99%,n=9),建立了十七烷二烯银杏酸(C17∶2)、氢化白果酸(C15∶0)相对定量分析方法。结论 该方法简便、准确、重现性好,可用于银杏叶的质量控制。不同产地、不同采收期的银杏叶药材中5种银杏酚酸类指标成分含量差异较大。
Abstract
OBJECTIVE To develop an HPLC method for simultaneous determination of ginkgolic acids(C13∶0,C15∶1,C17∶2,C15∶0,C17∶1) in Ginkgo Folium.METHODS The HPLC analysis was carried out on Phenomenex Luna C18(4.6 mm×250 mm, 5 μm) column with acetonitrile-0.1% phosphoric acid (90∶10)as mobile phase eluted at the flow rate of 1.0 mL·min-1. The detective wavelength was set at 310 nm, and the column temperature was set at 30 ℃. RESULTS The calibration curve was linear within the range of 1.47-29.40 μg·mL-1 for C13∶0,6.05-121.00 μg·mL-1 for C15∶1 and 8.00-160.00 μg·mL-1 for C17∶1, respectively. The average recoveries for the three marker compounds are 98.6%-100.1%. CONCLUSION This method is simple, accurate, and practical for the quality control of Ginkgo Folium. There are some differences in the contents of the five marker compounds from different producing areas and different collection periods.
关键词
银杏叶 /
白果新酸(C13∶0) /
白果酸(C15∶1) /
十七烷二烯银杏酸(C17∶2) /
氢化白果酸(C15∶0) /
十七烷一烯银杏酸(C17∶1) /
高效液相色谱法
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Key words
Ginkgo Folium /
ginkgolic acid (C13∶0) /
ginkgolic acid(C15∶1) /
C17∶2 /
ginkgolic acid(C15∶0) /
ginkgolic acid(C17∶1) /
HPLC
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中图分类号：
R248.1
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参考文献
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脚注
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基金
国家“重大新药创制”科技重大专项(2011ZX09201-201-012,2014ZX09201-022-004)
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